MAGE-Targeted Gold Nanoparticles for Ultrasound Imaging-Guided Phototherapy in Melanoma.
Xuelin LiShigen ZhongCuncheng ZhangPan LiHaitao RanZhi-Gang WangPublished in: BioMed research international (2020)
Gold nanorods exhibit a wide variety of applications such as tumor molecular imaging and photothermal therapy (PTT) due to their tunable optical properties. Several studies have demonstrated that the combination of other therapeutic strategies may improve PTT efficiency. A method called optical droplet vaporization (ODV) was considered as another noninvasive imaging and therapy strategy. Via the ODV method, superheated perfluorocarbon droplets can be vaporized to a gas phase for enhancing ultrasound imaging; meanwhile, this violent process can cause damage to cells and tissue. In addition, active targeting through the functionalization with targeting ligands can effectively increase nanoprobe accumulation in the tumor area, improving the sensitivity and specificity of imaging and therapy. Our study prepared a nanoparticle loaded with gold nanorods and perfluorinated hexane and conjugated to a monoclonal antibody (MAGE-1 antibody) to melanoma-associated antigens (MAGE) targeting melanoma, investigated the synergistic effect of PTT/ODV therapy, and monitored the therapeutic effect using ultrasound. The prepared MAGE-Au-PFH-NPs achieved complete eradication of tumors. Meanwhile, the MAGE-Au-PFH-NPs also possess significant ultrasound imaging signal enhancement, which shows the potential for imaging-guided tumor therapy in the future.
Keyphrases
- cancer therapy
- high resolution
- gold nanoparticles
- monoclonal antibody
- reduced graphene oxide
- magnetic resonance imaging
- drug delivery
- stem cells
- photodynamic therapy
- oxidative stress
- immune response
- high speed
- risk assessment
- single cell
- signaling pathway
- cell proliferation
- mesenchymal stem cells
- bone marrow
- skin cancer
- fluorescence imaging
- single molecule
- contrast enhanced ultrasound
- replacement therapy