Conformational Dynamics of Human ALKBH2 Dioxygenase in the Course of DNA Repair as Revealed by Stopped-Flow Fluorescence Spectroscopy.
Lyubov Yu KanazhevskayaDenis A SmyshliaevNadezhda A TimofeyevaAlexander A IshchenkoMurat K SaparbaevNikita A KuznetsovOlga S FedorovaPublished in: Molecules (Basel, Switzerland) (2022)
Elucidation of physicochemical mechanisms of enzymatic processes is one of the main tasks of modern biology. High efficiency and selectivity of enzymatic catalysis are mostly ensured by conformational dynamics of enzymes and substrates. Here, we applied a stopped-flow kinetic analysis based on fluorescent spectroscopy to investigate mechanisms of conformational transformations during the removal of alkylated bases from DNA by ALKBH2, a human homolog of Escherichia coli AlkB dioxygenase. This enzyme protects genomic DNA against various alkyl lesions through a sophisticated catalytic mechanism supported by a cofactor (Fe(II)), a cosubstrate (2-oxoglutarate), and O 2 . We present here a comparative study of conformational dynamics in complexes of the ALKBH2 protein with double-stranded DNA substrates containing N1-methyladenine, N3-methylcytosine, or 1,N6-ethenoadenine. By means of fluorescent labels of different types, simultaneous detection of conformational transitions in the protein globule and DNA substrate molecule was performed. Fitting of the kinetic curves by a nonlinear-regression method yielded a molecular mechanism and rate constants of its individual steps. The results shed light on overall conformational dynamics of ALKBH2 and damaged DNA during the catalytic cycle.
Keyphrases
- single molecule
- living cells
- dna repair
- escherichia coli
- endothelial cells
- circulating tumor
- molecular dynamics
- molecular dynamics simulations
- cell free
- quantum dots
- dna damage
- hydrogen peroxide
- induced pluripotent stem cells
- binding protein
- nucleic acid
- working memory
- dna methylation
- high resolution
- copy number
- sensitive detection
- nitric oxide
- fluorescent probe