Water-Soluble Glutamic Acid Derivatives Produced in Culture by Penicillium solitum IS1-A from King George Island, Maritime Antarctica.
Julie P G RodríguezDarlon I BernardiJuliana R GubianiJuliana Magalhães de OliveiraRaquel P Morais-UranoAriane F BertonhaKarin F BandeiraJairo I Q BullaLara D SetteAntonio G FerreiraJoao M BatistaThayná de Souza SilvaRaquel Alves Dos SantosCarlos Henrique Martins da SilvaSimone P LiraMarcos G da CunhaDaniela B B TrivellaNathalia GrazziaNatália E S GomesFernanda GadelhaDanilo C MiguelAna Carolina G CauzMarcelo BrocchiRoberto G S BerlinckPublished in: Journal of natural products (2020)
A new method of screening was developed to generate 770 organic and water-soluble fractions from extracts of nine species of marine sponges, from the growth media of 18 species of marine-derived fungi, and from the growth media of 13 species of endophytic fungi. The screening results indicated that water-soluble fractions displayed significant bioactivity in cytotoxic, antibiotic, anti-Leishmania, anti-Trypanosoma cruzi, and inhibition of proteasome assays. Purification of water-soluble fractions from the growth medium of Penicillium solitum IS1-A provided the new glutamic acid derivatives solitumine A (1), solitumine B (2), and solitumidines A-D (3-6). The structures of compounds 1-6 have been established by analysis of spectroscopic data, chemical derivatizations, and vibrational circular dichroism calculations. Although no biological activity could be observed for compounds 1-6, the new structures reported for 1-6 indicate that the investigation of water-soluble natural products represents a relevant strategy in finding new secondary metabolites.