Measuring the dynamic response of the thylakoid architecture in plant leaves by electron microscopy.

A methodical pipeline ranging from optimized leaf tissue preparation for electron microscopy to quantitative image analysis was established. This methodical development was employed to study details of light-induced changes in the plant thylakoid ultrastructure. It was found that the lumen of the entire thylakoid system (stacked and unstacked domains) undergoes light-induced swelling, whereas adjacent membranes on the stroma side in stacked grana thylakoid approach each other.