Nanopore sequencing as a scalable, cost-effective platform for analyzing polyclonal vector integration sites following clinical T cell therapy.
Ping ZhangDevika GanesamoorthySon Hoang NguyenRaymond AuLachlan J M CoinSiok-Keen TeyPublished in: Journal for immunotherapy of cancer (2021)
Our study shows that it is feasible to use nanopore sequencing to map polyclonal vector integration sites. The assay is scalable and requires minimum capital, which together enable cost-effective and timely analysis. Further refinement is required to reduce amplification bias and improve single nucleotide resolution.