Localization microscopy at doubled precision with patterned illumination.
Jelmer CnossenTaylor HinsdaleRasmus Ø ThorsenMarijn SiemonsFlorian SchuederRalf JungmannCarlas S SmithBernd RiegerSjoerd StallingaPublished in: Nature methods (2019)
MINFLUX offers a breakthrough in single molecule localization precision, but is limited in field of view. Here we combine centroid estimation and illumination pattern induced photon count variations in a conventional widefield imaging setup to extract position information over a typical micrometer-sized field of view. We show a near two-fold improvement in precision over standard localization with the same photon count on DNA-origami nanostructures and tubulin in cells, using DNA-PAINT and STORM imaging.
Keyphrases
- single molecule
- living cells
- high resolution
- atomic force microscopy
- induced apoptosis
- oxidative stress
- peripheral blood
- diabetic rats
- high glucose
- signaling pathway
- healthcare
- cell death
- endothelial cells
- photodynamic therapy
- cell proliferation
- endoplasmic reticulum stress
- high speed
- single cell
- optical coherence tomography