Comparative analysis of quantitative methodologies for Vibrionaceae biofilms.
Alba A Chavez-DozalNeda NourabadiMartina ErkenDiane McDougaldMichele K NishiguchiPublished in: Folia microbiologica (2016)
Multiple symbiotic and free-living Vibrio spp. grow as a form of microbial community known as a biofilm. In the laboratory, methods to quantify Vibrio biofilm mass include crystal violet staining, direct colony-forming unit (CFU) counting, dry biofilm cell mass measurement, and observation of development of wrinkled colonies. Another approach for bacterial biofilms also involves the use of tetrazolium (XTT) assays (used widely in studies of fungi) that are an appropriate measure of metabolic activity and vitality of cells within the biofilm matrix. This study systematically tested five techniques, among which the XTT assay and wrinkled colony measurement provided the most reproducible, accurate, and efficient methods for the quantitative estimation of Vibrionaceae biofilms.
Keyphrases
- candida albicans
- biofilm formation
- microbial community
- pseudomonas aeruginosa
- staphylococcus aureus
- high resolution
- high throughput
- induced apoptosis
- antibiotic resistance genes
- single cell
- cystic fibrosis
- escherichia coli
- stem cells
- cell cycle arrest
- cell death
- cell proliferation
- oxidative stress
- wastewater treatment