Dynamic full-field optical coherence tomography: 3D live-imaging of retinal organoids.
Jules SchollerKassandra GrouxOlivier GoureauJosé-Alain SahelMathias FinkSacha ReichmanClaude BoccaraKate GrievePublished in: Light, science & applications (2020)
Optical coherence tomography offers astounding opportunities to image the complex structure of living tissue but lacks functional information. We present dynamic full-field optical coherence tomography as a technique to noninvasively image living human induced pluripotent stem cell-derived retinal organoids. Coloured images with an endogenous contrast linked to organelle motility are generated, with submicrometre spatial resolution and millisecond temporal resolution, creating a way to identify specific cell types in living tissue via their function.
Keyphrases
- optical coherence tomography
- diabetic retinopathy
- induced pluripotent stem cells
- deep learning
- optic nerve
- endothelial cells
- single molecule
- high glucose
- high resolution
- magnetic resonance
- single cell
- cell therapy
- cystic fibrosis
- computed tomography
- diabetic rats
- stem cells
- escherichia coli
- pseudomonas aeruginosa
- contrast enhanced
- mesenchymal stem cells
- photodynamic therapy