Colorimetric loop-mediated isothermal amplification (LAMP) for cost-effective and quantitative detection of SARS-CoV-2: the change in color in LAMP-based assays quantitatively correlates with viral copy number.
Everardo González-GonzálezItzel Montserrat Lara-MayorgaIram Pablo Rodríguez-SánchezYu Shrike ZhangSergio Omar Martínez-ChapaGrissel Trujillo-de SantiagoMario Moisés ÁlvarezPublished in: Analytical methods : advancing methods and applications (2021)
We demonstrate a loop-mediated isothermal amplification (LAMP) method to detect and amplify SARS-CoV-2 genetic sequences using a set of in-house designed initiators that target regions encoding the N protein. We were able to detect and amplify SARS-CoV-2 nucleic acids in the range of 62 to 2 × 105 DNA copies by this straightforward method. Using synthetic SARS-CoV-2 samples and RNA extracts from patients, we demonstrate that colorimetric LAMP is a quantitative method comparable in diagnostic performance to RT-qPCR (i.e., sensitivity of 92.85% and specificity of 81.25% in a set of 44 RNA extracts from patients analyzed in a hospital setting).
Keyphrases
- loop mediated isothermal amplification
- sars cov
- sensitive detection
- copy number
- end stage renal disease
- respiratory syndrome coronavirus
- newly diagnosed
- chronic kidney disease
- ejection fraction
- prognostic factors
- genome wide
- peritoneal dialysis
- mitochondrial dna
- hydrogen peroxide
- quantum dots
- high resolution
- emergency department
- coronavirus disease
- gene expression
- patient reported outcomes