Heterogeneity and genomic loci of ubiquitous transgenic Cre reporter lines in zebrafish.

Robert L Lalonde Cassie L Kemmler Frederike W RiemslaghAndrew J AmanJelena Kresoja-Rakic Hannah R MoranSusan NieuwenhuizeDavid M ParichyAlexa Burger Christian Mosimann

Published in: Developmental dynamics : an official publication of the American Association of Anatomists (2022)

Our data documents the heterogeneity among lox-based Switch transgenes toward informing suitable transgene selection for lineage labeling experiments. Our work further proposes that ubi:Switch and hsp70l:Switch define genomic integration sites suitable for universal transgene or switch reporter knock-in in zebrafish.

Keyphrases

single cell
crispr cas
heat shock protein
genome wide
heat stress
machine learning
gene expression
dna methylation
data analysis