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Optimal control theory enables homonuclear decoupling without Bloch-Siegert shifts in NMR spectroscopy.

Paul W CooteScott A RobsonAbhinav DubeyAndras BoeszoermenyiMengxia ZhaoGerhard WagnerHaribabu Arthanari
Published in: Nature communications (2018)
The Bloch-Siegert shift is a phenomenon in NMR spectroscopy and atomic physics in which the observed resonance frequency is changed by the presence of an off-resonance applied field. In NMR, it occurs especially in the context of homonuclear decoupling. Here we develop a practical method for homonuclear decoupling that avoids inducing Bloch-Siegert shifts. This approach enables accurate observation of the resonance frequencies of decoupled nuclear spins. We apply this method to increase the resolution of the HNCA experiment. We also observe a doubling in sensitivity for a 30 kDa protein. We demonstrate the use of band-selective Cβ decoupling to produce amino acid-specific line shapes, which are valuable for assigning resonances to the protein sequence. Finally, we assign the backbone of a 30 kDa protein, Human Carbonic Anhydrase II, using only HNCA experiments acquired with band-selective decoupling schemes, and instrument time of one week.
Keyphrases
  • amino acid
  • energy transfer
  • protein protein
  • magnetic resonance
  • high resolution
  • heat shock protein
  • binding protein
  • clinical trial
  • small molecule