Evidence supporting a role for the immune checkpoint protein B7-H3 in NK cell-mediated cytotoxicity against AML.
Anudishi TyagiStanley LyFouad El-DanaBin YuanAppalaraju JaggupilliSabrina GrimmMarina Y KonoplevaHans-Jörg BühringVenkata Lokesh BattulaPublished in: Blood (2022)
We observed that the immune checkpoint protein B7-H3 is overexpressed in acute myeloid leukemia (AML) patients with poor treatment outcomes. Inhibition of B7-H3 expression or blocking of its activity using a novel monoclonal antibody (T-1A5) in AML cells significantly enhanced natural killer (NK) cell-mediated cytotoxicity in AML cells in vitro and in vivo. Moreover, a human-mouse chimera of this antibody (ChT-1A5) induced antibody-dependent cell-mediated cytotoxicity (ADCC) in B7-H3+ primary AML cells, but not in normal hematopoietic cells, suggesting the specify of this antibody for AML cells. Epitope mapping studies identified that both T-1A5 and ChT-1A5 antibodies bind to the FG-loop region of B7-H3, which is known to regulate the immunosuppressive function of B7-H3. Furthermore, treatment with ChT-1A5 in combination with human NK cells significantly prolonged survival in AML patient-derived xenograft (PDX) models. Our results suggest that the ChT-1A5 antibody can inhibit the immunosuppressive function of B7-H3 protein as well as induce ADCC in B7-H3+ AML.
Keyphrases
- acute myeloid leukemia
- induced apoptosis
- nk cells
- cell cycle arrest
- monoclonal antibody
- endothelial cells
- cell death
- endoplasmic reticulum stress
- signaling pathway
- oxidative stress
- high resolution
- transcription factor
- single cell
- small molecule
- stem cells
- high glucose
- pi k akt
- cell therapy
- mesenchymal stem cells
- free survival