Comprehensive Analysis of Mouse Hippocampal Lysine Acetylome Mediated by Sea Cucumber Peptides Preventing Memory Impairment.
Zhiqiang LuXiaomeng XuDongmei LiNa SunSongyi LinPublished in: Journal of agricultural and food chemistry (2021)
Memory impairment is becoming a potential health issue with the delicacy of diet and social stress. Sea cucumber peptides (SCP) prevent memory impairment, as previously reported. In this study, further research was performed using hippocampal lysine-acetylome to explore molecular regulation mechanisms. C57BL/6 mice were treated with scopolamine via intraperitoneal injection to simulate memory impairment. To determine the influence of SCP on the total acetylated-protein level of the hippocampus, acetylated-proteomics was performed. SCP increased the acetylation level of histone (H3 and H4). Meanwhile, for non-histones, the differentially acetylated proteins were involved in multiple memory-related pathways, as shown by KEGG enrichment analysis. Additionally, long-term potentiation was confirmed by western blotting. Finally, a combined analysis of proteome and lysine acetylome revealed that SCP contributed to synaptic vesicle cycle regulation and dopamine metabolism. Consequently, our findings revealed that SCP was potentially neuroprotective by regulating post-transcriptional hippocampal protein acetylation.
Keyphrases
- working memory
- amino acid
- cerebral ischemia
- healthcare
- mental health
- gene expression
- public health
- single cell
- transcription factor
- protein protein
- small molecule
- histone deacetylase
- oxidative stress
- single molecule
- uric acid
- skeletal muscle
- prefrontal cortex
- health information
- newly diagnosed
- heat stress
- stress induced