POLB Regulates Proliferation and Apoptosis of Bovine Primary Myocytes.
Geyang ZhangJiamei WangYulong LiZijing ZhangXiangnan WangFuying ChenQiaoting ShiYongzhen HuangEryao WangShijie LyuPublished in: Animals : an open access journal from MDPI (2024)
DNA polymerase β (DNA polymerase beta ( POLB )) belongs to a member of the DNA polymerase X family, mainly involved in various biological metabolic processes, such as eukaryotic DNA replication, DNA damage repair, gene recombination, and cell cycle regulation. In this study, the muscle development-related gene POLB was screened by selection signature and RNA-seq analysis and then validated for the proliferation and apoptosis of bovine primary myocytes. It was also found that overexpression of the POLB gene had a pro-apoptosis effect, but interfering with the expression of the gene had no significant effect on cells. Then, the analysis of related apoptotic genes revealed that POLB overexpression affected CASP9 gene expression.
Keyphrases
- cell cycle arrest
- cell cycle
- cell death
- genome wide
- dna damage
- oxidative stress
- genome wide identification
- rna seq
- gene expression
- endoplasmic reticulum stress
- cell proliferation
- copy number
- circulating tumor
- single cell
- induced apoptosis
- cell free
- single molecule
- signaling pathway
- dna methylation
- transcription factor
- pi k akt
- genome wide analysis
- dna repair
- skeletal muscle
- structural basis
- nucleic acid