Cryopreservation of mesenchymal stem cells derived from dental pulp: a systematic review.
Sabrina Moreira PaesYasmine Mendes PupoBruno Cavalini CavenagoThiago Fonseca-SilvaCarolina Carvalho de Oliveira SantosPublished in: Restorative dentistry & endodontics (2021)
The cryopreservation process must be carried out in 6 stages: tooth disinfection, pulp extraction, cell isolation, cell proliferation, cryopreservation, and thawing. In addition, it can be inferred that the use of dimethyl sulfoxide, programmable freezing, and storage in liquid nitrogen are associated with a high rate of cell viability after thawing and a high rate of cell proliferation in both primary and permanent teeth.