Molecular signature of progenitor cells isolated from young and adult human hearts.
Ann-Sophie WalravensMaarten VanhaverbekeLara OttavianiHilde GillijnsSander TrensonNina Vanden DriesscheAernout LuttunBart MeynsPaul HerijgersFilip RegaRuth HeyingMaurilio SampaolesiStefan JanssensPublished in: Scientific reports (2018)
The loss of endogenous cardiac regenerative capacity within the first week of postnatal life has intensified clinical trials to induce cardiac regeneration in the adult mammalian heart using different progenitor cell types. We hypothesized that donor age-related phenotypic and functional characteristics of cardiac progenitor cells (CPC) account for mixed results of cell-based cardiac repair. We compared expression profiles and cell turnover rates of human heart-derived c-kitpos progenitors (c-kitpos CPC) and cardiosphere-derived cells (CDC) from young and adult donor origin and studied their in vitro angiogenic and cardiac differentiation potential, which can be relevant for cardiac repair. We report that 3-dimensional CDC expansion recapitulates a conducive environment for growth factor and cytokine release from adult donor cells (aCDC) that optimally supports vascular tube formation and vessel sprouting. Transdifferentiation capacity of c-kitpos CPCs and CDCs towards cardiomyocyte-like cells was modest, however, most notable in young c-kitpos cells and adult CDCs. Progenitors isolated with different methods thus show cell- and donor-specific characteristics that may account for variable contributions in functional myocardial recovery.
Keyphrases
- left ventricular
- induced apoptosis
- growth factor
- cell therapy
- clinical trial
- stem cells
- single cell
- cell cycle arrest
- heart failure
- endothelial cells
- childhood cancer
- mesenchymal stem cells
- middle aged
- risk assessment
- atrial fibrillation
- oxidative stress
- induced pluripotent stem cells
- cell proliferation
- pi k akt
- double blind
- phase ii
- phase iii