Construction of Porous Perovskite Oxide Microrods with Au Nanoparticle Anchor for Precise Metabolic Diagnosis of Alzheimer's Disease.

Alzheimer's disease (AD) is a progressive illness, and early diagnosis and treatment can help delay its progression. However, clinics still lack high-throughput, low-invasive, precise and objective diagnostic strategies. Here, we develop the Au nanoparticles anchored porous perovskite oxide microrods (CTO@Au) with designed superior properties to construct a high-throughput detection platform. Specifically, we can obtain a single metabolic fingerprinting from only 30 nL of serum within seconds, enabling the rapid acquisition of 239 × 8 high-quality fingerprints in about 2 hours. We distinguish AD from health controls and Parkinson's disease with an area under the curve (AUC) of 1.000. Moreover, we identify eight specific metabolites as a biomarker panel, based on which we achieve precise diagnosis of AD, with an AUC of 1.000 in blind test. We also investigate and discuss the possible relevant pathways and potential mechanism involved in these biomarkers. Our work provides a high-performance platform for metabolic diagnostic analysis. This article is protected by copyright. All rights reserved.