Modulation of Klebsiella pneumoniae Outer Membrane Vesicle Protein Cargo under Antibiotic Treatment.
Aline Castro Rodrigues LucenaMariana Galvão FerrariniWillian Klassen OliveiraBruna Hilzendeger MarconLuis Gustavo MorelloLysangela Ronalte AlvesHelisson FaoroPublished in: Biomedicines (2023)
Klebsiella pneumoniae is a nosocomial pathogen and an important propagator of multidrug-resistant (MDR) and extensively drug-resistant (XDR) strains. Like other Gram-negative bacteria, they secrete outer membrane vesicles (OMVs) that distribute virulence and resistance factors. Here, we subjected a K . pneumoniae -XDR to subinhibitory concentrations of meropenem, amikacin, polymyxin B, and a combination of these agents to evaluate changes in the protein cargo of OMVs through liquid chromatography-tandem mass spectrometry (LC-MS/MS). Genome sequencing of the clinical isolate K . pneumoniae strain HCD1 (KpHCD1) revealed the presence of 41 resistance genes and 159 virulence factors. We identified 64 proteins in KpHCD1-OMVs modulated with different antibiotic treatments involved in processing genetic information, environmental information, cell envelope formation, energy metabolism, and drug resistance. The OMV proteome expression profile suggests that OMVs may be associated with pathogenicity, survival, stress response, and resistance dissemination.
Keyphrases
- multidrug resistant
- drug resistant
- klebsiella pneumoniae
- gram negative
- acinetobacter baumannii
- liquid chromatography tandem mass spectrometry
- escherichia coli
- single cell
- biofilm formation
- pseudomonas aeruginosa
- genome wide
- staphylococcus aureus
- simultaneous determination
- antimicrobial resistance
- health information
- healthcare
- protein protein
- stem cells
- solid phase extraction
- amino acid
- binding protein
- dna methylation
- social media
- combination therapy
- mesenchymal stem cells
- gene expression
- copy number
- risk assessment
- mass spectrometry
- bioinformatics analysis
- climate change
- replacement therapy