Nano materials are utilized to improve the performance of some pharmaceuticals and materials, as well as to lessen the collateral damage they cause. The purpose of this study was to look at the effect of silver nanoparticles (AgNPs) produced by bacteria on some trophozoites of intestinal parasites. The silver nanoparticles were synthesized using Bacillus cereus (BAgNPs) and Chromobacterium violaceum (ChAgNPs) bacteria. The AgNPs production was confirmed by several tests and techniques, such as electron microscopy. The results of the analysis showed that the size of these particles was within the range of 21-96.71 nm for both BAgNPs and ChAgNPs. In vitro and in vivo efficacy were tested on some trophozoites of intestinal parasites. The effect of the AgNPs on Entamoeba histolytica trophozoites in culture was significantly higher compared to metronidazole. The highest percentage of inhibition was 70.6% and 76.5% for the particles prepared from B. cereus and C. violaceum without significant differences between the two bacteria, compared to 57.6% inhibition for metronidazole. The in vivo effect of the AgNPs on Giardia lamblia exceeded that of metronidazole and led to the total disappearance of the stages from mice faeces after 3-4 days. Likewise, Tritrichomonas muris numbers were also reduced in infected mice treated with AgNPs, with the highest inhibition rate of 81.3%. From above can concluded these bacterially produced nanoparticles have proven strong efficacy, and it is possible to recommend their use independently after conducting studies on the extent of their effects on the body and proving their safety.