Lignocellulosic biomass is an attractive sustainable carbon source for fermentative production of bioethanol. In this context, use of microbial consortia consisting of substrate-selective microbes is advantageous as it eliminates the negative impacts of glucose catabolite repression. In this study, a detailed in silico analysis of bioethanol production from glucose-xylose mixtures of various compositions by coculture fermentation of xylose-selective Escherichia coli strain ZSC113 and glucose-selective wild-type Saccharomyces cerevisiae is presented. Dynamic flux balance models based on available genome-scale metabolic networks of the microorganisms have been used to analyze bioethanol production and the maximization of ethanol productivity is addressed by computing optimal aerobic-anaerobic switching times. A set of genetic engineering strategies for ethanol overproduction by E. coli strain ZSC113 have been evaluated for their efficiency in the context of batch coculture process. Finally, simulations are carried out to determine the pairs of genetically modified E. coli strain ZSC113 and S. cerevisiae that significantly enhance ethanol productivity in batch coculture fermentation.
Keyphrases
- saccharomyces cerevisiae
- escherichia coli
- wild type
- anaerobic digestion
- microbial community
- blood glucose
- climate change
- wastewater treatment
- molecular docking
- genome wide
- ionic liquid
- gene expression
- biofilm formation
- adipose tissue
- metabolic syndrome
- type diabetes
- blood pressure
- cystic fibrosis
- molecular dynamics simulations
- amino acid
- heavy metals