Single-cell RNA sequencing of cells from fresh or frozen tissue reveals a signature of freezing marked by heightened stress and activation.
Christopher T StamperAnne MarchalotChristopher A TibbittWhitney WeigelMattias JangardJakob TheorellJenny MjösbergPublished in: European journal of immunology (2024)
Thawing of viably frozen human tissue T cells, ILCs, and NK cells and subsequent single-cell RNA sequencing reveals that recovery of cellular subclusters is variably impacted. While freeze-thawing does not alter the transcriptional profiles of cells, it upregulates genes and gene pathways associated with stress and activation.
Keyphrases
- single cell
- rna seq
- nk cells
- high throughput
- induced apoptosis
- genome wide
- endothelial cells
- genome wide identification
- cell cycle arrest
- gene expression
- stress induced
- copy number
- genome wide analysis
- induced pluripotent stem cells
- cell death
- cell proliferation
- endoplasmic reticulum stress
- signaling pathway
- oxidative stress