AIEgen Nanoparticles of Arylamino Fumaronitrile Derivative with High Near-Infrared Emission for Two-Photon Imaging and in Vivo Cell Tracking.
Zhonghua LiuFang LiuYijian GaoWeixia QingYongwei HuangShengliang LiDongzhu JinPublished in: ACS applied bio materials (2018)
Developing of two-photon materials for live-cell imaging and in vivo analysis in-depth have received great attention, and it is urgent so that such microscopy techniques could be promoted and advanced using the powerful probes. Herein, a new arylamino fumaronitrile derivative NPAPF was synthesized and transferred as aggregation-induced emission luminogen (AIEgen) fluorescent nanoparticles (AF-NPs) via assembly technique. This AF-NP exhibited a two-photon absorption cross-section at 2.6 × 10 6 GM with 19.5% of fluorescence quantum yield. Moreover, utilizing the great potential of AF-NPs, two-photon imaging of live cells with good cytocompatibility is realized upon two-photon microscopy. By in vivo long-term tracing studies of mesenchymal stem cells, we demonstrated the tremendous advantage of AF-NPs tracer in monitoring the stem cells transplant. Therefore, our unique AF-NPs provided an efficient two-photon-absorbing probe for investigating biological mechanism and behavior, and opened a new avenue for spatiotemporal visualization of transplanted stem cells.
Keyphrases
- living cells
- single molecule
- stem cells
- high resolution
- atrial fibrillation
- fluorescent probe
- mesenchymal stem cells
- monte carlo
- cell therapy
- oxide nanoparticles
- optical coherence tomography
- single cell
- small molecule
- high throughput
- fluorescence imaging
- quantum dots
- high speed
- molecular dynamics
- bone marrow
- photodynamic therapy
- signaling pathway
- oxidative stress
- pi k akt
- risk assessment
- cell proliferation
- computed tomography
- climate change
- umbilical cord
- pet ct
- pet imaging