Thalassosamide, a Siderophore Discovered from the Marine-Derived Bacterium Thalassospira profundimaris.
Fan ZhangKenneth BarnsF Michael HoffmannDoug R BraunDavid R AndesTim S BugniPublished in: Journal of natural products (2017)
Here we describe the rapid identification and prioritization of novel active marine natural products using an improved dereplication strategy. During the course of our screening of marine natural product libraries, a new cyclic trihydroxamate compound, thalassosamide, was discovered from the α-proteobacterium Thalassospira profundimaris. Its structure was determined by 2D NMR and MS/MS experiments, and the absolute configuration of the lysine-derived units was established by Marfey's analysis, whereas that of C-9, 9', and 9″ was determined via the circular dichroism data of the [Rh2(OCOCF3)4] complex and DFT NMR calculations. Thalassosamide showed moderate in vivo efficacy against Pseudomonas aeruginosa.
Keyphrases
- pseudomonas aeruginosa
- ms ms
- magnetic resonance
- density functional theory
- high resolution
- solid state
- cystic fibrosis
- molecular dynamics
- electronic health record
- molecular dynamics simulations
- high intensity
- big data
- biofilm formation
- machine learning
- data analysis
- crystal structure
- loop mediated isothermal amplification
- tandem mass spectrometry