The protective effects of topiramate on intestinal injury induced with infrarenal aortic occlusion via oxidative stress and apoptosis.
Ahmet PergelGökhan DemiralLevent TumkayaTolga MercantepeAli ÖzdemirSüleyman KalcanMuhammed Kadri ÇolakoğluAdnan YılmazRecep BedirAhmet KarakayaPublished in: Clinical and experimental hypertension (New York, N.Y. : 1993) (2021)
Purpose: Prolonged surgical procedures and some clinical conditions such as surgeries of thoracoabdominal aorta, mesenteric ischemia, cardiopulmonary bypass, strangulated hernias and neonatal necrotizing enterocolitis may cause decreased perfusion and injury of relevant organs and tissues. After reperfusion, injuries may get worse, leading to ischemia-reperfusion (I/R) injury. Reperfusion following arterial clamping allows oxygen to ischemic tissues and produce injury by multiple mechanisms, including neutrophilic infiltration, intracellular adhesion molecules, and generation of reactive oxygen radicals. In this study with the analysis of SOD, MDA and Caspase-3 levels, we aimed to investigate the effect of topiramate on the outcome of I/R occured after abdominal aorta clamping on rats.Materials and Methods: Totaly 24 Sprague-Dawley male rats were randomly divided into three experimental groups; the control group (n = 8), I/R (n = 8) and I/R+ topiramate (n = 8). Topiramate (100 mg/kg/day); 50 mg/kg (single dose) was administered intraperitoneally after being diluted with saline 5 days before I/R.Results: The intestinal tissue of the ischemia group displayed hemorrhage, Crypts of Lieberkuhn degeneration, ulceration, vascular congestion and edematous fields as a result of aortic occlusion. We also observed that MDA levels and Caspase-3 positivity increased and SOD levels decreased in the small intestine. However, topiramate administration decreased Crypts of Lieberkuhn degeneration, ulceration, vascular congestion and edematous fields, Caspase-3 positivity, and MDA levels.Conclusion: Our findings suggest that topiramate is effective against aortic occlusion-induced intestinal injury by reducing oxidative stress and apoptosis.
Keyphrases
- oxidative stress
- diabetic rats
- aortic valve
- cell death
- cell cycle arrest
- induced apoptosis
- pulmonary artery
- endoplasmic reticulum stress
- breast cancer cells
- gene expression
- cerebral ischemia
- ischemia reperfusion injury
- left ventricular
- dna damage
- aortic dissection
- acute myocardial infarction
- coronary artery
- drug induced
- heart failure
- escherichia coli
- signaling pathway
- coronary artery disease
- amyotrophic lateral sclerosis
- atrial fibrillation
- acute coronary syndrome
- reactive oxygen species
- staphylococcus aureus
- brain injury
- contrast enhanced