The combinatory effect of scaffold topography and culture condition: an approach to nucleus pulposus tissue engineering.
Noviana VanawatiAnggraini BarlianHermawan JudawisastraIndra WibowoPublished in: Future science OA (2022)
The results indicated that SF scaffold with a minimum thickness 3.5 mm and pore diameter of 500 μm with cells cultured under low glucose, 10% PRP and normoxia conditions induced the growth and differentiation of hWJ-MSCs, indicated by the accumulation of glycosaminoglycans content and the presence of type II collagen, as markers of NP-like cells.
Keyphrases
- tissue engineering
- induced apoptosis
- cell cycle arrest
- high glucose
- mesenchymal stem cells
- endothelial cells
- diabetic rats
- optical coherence tomography
- blood glucose
- signaling pathway
- endoplasmic reticulum stress
- umbilical cord
- cell death
- oxidative stress
- optic nerve
- metabolic syndrome
- type diabetes
- adipose tissue
- blood pressure
- bone marrow