Amoxicillin and gentamicin antibiotics treatment adversely influence the fertility and morphology through decreasing the Dazl gene expression level and increasing the oxidative stress.
Muhammet İhsan KaramanHarun BudakMehmet ÇiftciPublished in: Archives of physiology and biochemistry (2018)
The present study was designed to explain the impact of amoxicillin, gentamicin, and cefazolin on the oxidative stress (OS) and reproductivity in the mouse testes. Our data showed that reduced glutathione (GSH) level, which is a marker for OS, strikingly reduced and hydrogen peroxide (H2O2) level, which acts as a signaling molecule in mammalian germ cells, strikingly increased with amoxicillin, gentamicin, and cefazolin treatment. The gene expression and enzymatic activity of superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), glucose-6-phosphate dehydrogenase (G6PD), and glutathione-S-transferases (GST) were significantly affected in the presence of these antibiotics. Also, spermatogenesis was adversely affected by suppressing Deleted in Azoospermia (Dazl) gene expression. Finally, oxidative stress and spermatogenesis failure distorted to sperm viability, motility, and morphology in amoxicillin and gentamicin-treated mice.
Keyphrases
- gene expression
- hydrogen peroxide
- oxidative stress
- induced apoptosis
- dna methylation
- dna damage
- nitric oxide
- ischemia reperfusion injury
- diabetic rats
- endoplasmic reticulum stress
- signaling pathway
- adipose tissue
- combination therapy
- blood glucose
- big data
- escherichia coli
- staphylococcus aureus
- cell cycle arrest
- artificial intelligence
- data analysis