To investigate the differential immunology in Ningxiang and Berkshire pigs and their F 1 offspring (F 1 offspring), physiological and biochemical indicators in the plasma and spleen were analyzed. Then, transcriptomic analysis of the spleen identified 1348, 408, and 207 differentially expressed genes (DEGs) in comparisons of Ningxiang vs. Berkshire, Berkshire vs. F 1 offspring, and Ningxiang vs. F 1 offspring, respectively. In Ningxiang vs. Berkshire pigs, the gene ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis indicated that the DEGs included CD163 , MARCO , CXCL14 , CCL19 , and PPBP , which are associated with immunity. GO and KEGG analyses were also conducted comparing F 1 offspring and their parents. The DEGs, including BPIFB1 , HAVCR2 , CD163 , DDX3X , CCR5 , and ITGB3 , were enriched in immune-related pathways. Protein-protein interaction (PPI) analysis indicated that the EGFR and ITGA2 genes were key hub genes. In conclusion, this study identifies significant immune DEGs in different pig breeds, providing data to support the exploration of breeding strategies for disease resistance in local and crossbred pig populations.
Keyphrases
- genome wide
- high fat diet
- genome wide identification
- protein protein
- bioinformatics analysis
- gene expression
- dna methylation
- small molecule
- small cell lung cancer
- copy number
- genetic diversity
- dendritic cells
- machine learning
- epidermal growth factor receptor
- transcription factor
- immune response
- nk cells
- single cell
- liver injury