Near infrared-responsive quinacrine-gold hybrid nanoparticles deregulate HSP-70/P300-mediated H3K14 acetylation in ER/PR+ breast cancer stem cells.
Somya Ranjan DashChinmay DasBiswajit DasAtala Bihari JenaSubarno PaulSaptarshi SinhaJasaswini TripathyChanakya Nath KunduPublished in: Nanomedicine (London, England) (2024)
Aim: This study aimed to determine if quinacrine-gold hybrid nanoparticles (QAuNPs) + near-infrared (NIR) deregulate HSP-70/P300 complex-mediated H3K14 acetylation in estrogen receptor/progesterone receptor (ER/PR+) breast cancer stem cells (CSCs). Materials & methods: Various cells and mouse-based systems were used as models. Results: QAuNP + NIR treatment reduced the nuclear translocation of HSP-70, affected the histone acetyltransferase activity of P300 and specifically decreased H3K14 acetylation in ER/PR+ breast CSCs. Finally, HSP-70 knockdown showed a reduction in P300 histone acetyltransferase activity, decreased H3K14 acetylation and inhibited activation of the TGF-β gene. Conclusion: This study revealed that QAuNP + NIR irradiation inhibits oncogenic activation of the TGF-β gene by decreasing H3K14 acetylation mediated through the HSP-70/P300 nuclear complex in ER/PR+ breast CSCs.
Keyphrases
- cancer stem cells
- estrogen receptor
- heat shock protein
- heat shock
- heat stress
- histone deacetylase
- photodynamic therapy
- dna methylation
- endoplasmic reticulum
- transforming growth factor
- breast cancer cells
- fluorescence imaging
- genome wide
- copy number
- fluorescent probe
- single cell
- radiation induced
- signaling pathway
- smoking cessation
- binding protein