Anti-Stress and Anti-ROS Effects of MnOx-Functionalized Thermosensitive Nanohydrogel Protect BMSCs for Intervertebral Disc Degeneration Repair.
Xiaoxiong WangLiyang YuJiazhi DuanMingzheng ChangMin HaoZiqian XiangCheng QiuJunyuan SunDerun DiHe XiaDezheng LiSuomao YuanYonghao TianJichuan QiuHong LiuXinyu LiuYuanhua SangLianlei WangPublished in: Advanced healthcare materials (2024)
Stem cell transplantation is proven to be a promising strategy for intervertebral disc degeneration (IDD) repair. However, replicative senescence of bone marrow-derived mesenchymal stem cells (BMSCs), shear damage during direct injection, mechanical stress, and the reactive oxygen species (ROS)-rich microenvironment in degenerative intervertebral discs (IVDs) cause significant cellular damage and limit the therapeutic efficacy. Here, an injectable manganese oxide (MnOx)-functionalized thermosensitive nanohydrogel is proposed for BMSC transplantation for IDD therapy. The MnOx-functionalized thermosensitive nanohydrogel not only successfully protects BMSCs from shear force and mechanical stress before and after injection, but also repairs the harsh high-ROS environment in degenerative IVDs, thus effectively increasing the viability of BMSCs and resident nucleus pulposus cells (NPCs). The MnOx-functionalized thermosensitive nanohydrogel provides mechanical protection for stem cells and helps to remove endogenous ROS, providing a promising stem cell delivery platform for the treatment of IDD.
Keyphrases
- reactive oxygen species
- stem cells
- stem cell transplantation
- dna damage
- quantum dots
- cell death
- bone marrow
- stress induced
- high dose
- molecularly imprinted
- oxidative stress
- cell therapy
- induced apoptosis
- cell cycle arrest
- endothelial cells
- ultrasound guided
- low dose
- patient safety
- heat stress
- cell proliferation
- high throughput
- high resolution
- signaling pathway
- tissue engineering
- endoplasmic reticulum stress
- smoking cessation
- tandem mass spectrometry