Effects of poor sleep on the immune cell landscape as assessed by single-cell analysis.
Xiuxing LiuBinyao ChenZhaohao HuangRunping DuanHe LiLihui XieRong WangZhaohuai LiYuehan GaoYing-Feng ZhengWenru SuPublished in: Communications biology (2021)
Poor sleep has become an important public health issue. With loss of sleep durations, poor sleep has been linked to the increased risks for diseases. Here we employed mass cytometry and single-cell RNA sequencing to obtain a comprehensive human immune cells landscape in the context of poor sleep, which was analyzed in the context of subset composition, gene signatures, enriched pathways, transcriptional regulatory networks, and intercellular interactions. Participants subjected to staying up had increased T and plasma cell frequency, along with upregulated autoimmune-related markers and pathways in CD4+ T and B cells. Additionally, staying up reduced the differentiation and immune activity of cytotoxic cells, indicative of a predisposition to infection and tumor development. Finally, staying up influenced myeloid subsets distribution and induced inflammation development and cellular senescence. These findings could potentially give high-dimensional and advanced insights for understanding the cellular and molecular mechanisms of pathologic conditions related to poor sleep.
Keyphrases
- single cell
- rna seq
- sleep quality
- physical activity
- public health
- high throughput
- endothelial cells
- bone marrow
- oxidative stress
- multiple sclerosis
- induced apoptosis
- acute myeloid leukemia
- neoadjuvant chemotherapy
- diabetic rats
- copy number
- genome wide
- immune response
- cell therapy
- high glucose
- radiation therapy
- pi k akt
- data analysis
- induced pluripotent stem cells
- heat stress
- cell adhesion
- heat shock protein
- nk cells
- heat shock