Improving Enzyme Catalytic Efficiency by Co-operative Vibrational Strong Coupling of Water.

Here, we report enhancement of catalytic efficiency of an enzymatic reaction by co-operative vibrational strong coupling (VSC) of water and the enzyme α-chymotrypsin. Selective strong coupling of the O-H stretching mode of water along with O-H and N-H stretching modes of the enzyme modify the rate of the enzymatic ester hydrolysis, increasing the catalytic efficiency by more than 7 times. This is specifically achieved by controlling the rate-determining proton-transfer process through a co-operative mechanism. Here, VSC is also used as a spectroscopic tool to understand the mechanism of the enzymatic reaction, suggesting its potential applications in chemistry.