Facile Impedimetric Analysis of Neuronal Exosome Markers in Parkinson's Disease Diagnostics.
Ying FuCheng JiangGeorge K TofarisJason J DavisPublished in: Analytical chemistry (2020)
The egress of α-synuclein in neuronally derived exosomes predates the clinical presentation of Parkinson's disease (PD), offering a means of developing a predictive or prognostic test. Here, we report the reagentless impedimetric assay of two internal exosome markers (α-synuclein and syntenin-1) from neuronal exosomes. Exosomes were efficiently extracted from patient sera using anti-L1CAM conjugated zwitterionic polymer-modified magnetic beads prior to lysis and analyzed by electrochemical impedance spectroscopy. The quantification of α-synuclein level across 40 clinical samples resolved statistically significant differences between PD patients and healthy controls (HC).
Keyphrases
- mesenchymal stem cells
- stem cells
- end stage renal disease
- label free
- ejection fraction
- newly diagnosed
- chronic kidney disease
- molecularly imprinted
- peritoneal dialysis
- photodynamic therapy
- cerebral ischemia
- magnetic resonance imaging
- case report
- patient reported outcomes
- bone marrow
- ionic liquid
- reduced graphene oxide
- patient reported
- tandem mass spectrometry
- visible light