Indole alkaloid glycosides from Isatis tinctoria roots.
Dong-Dong ZhangKang DuYitian ZhaoSongshan ShiYingchun WuQi JiaKaixian ChenYi-Ming LiRui WangPublished in: Natural product research (2019)
Isatindigoside A and B (1 - 2), two new indole alkaloid glycosides along with five known ones (3 - 7) were obtained from the roots of I. tinctoria. Their structures were determined as isatindigoside A (1), isatindigoside B (2), isatindosulfonicacid A 3-O-β-D-glucopyranoside (3), indole-3-acetonitrile 6-O-β-D-glucopyranoside (4), isatindigobisindoloside A (5), isatindigobisindoloside B (6) isatindigobisindoloside F (7), by physicochemical properties and spectroscopic methods including 1 D, 2 D NMR, IR, HR-ESI-MS data. Nitric oxide (NO) inhibitory activities of all of the isolated compounds (1 - 7) were also evaluated. Compounds 2 and 7 showed inhibitory effects against LPS-stimulated RAW 264.7 cells with IC50 values of 27.6 μM and 18.8 μM, respectively.
Keyphrases
- nitric oxide
- ms ms
- induced apoptosis
- high resolution
- mass spectrometry
- magnetic resonance
- cell cycle arrest
- multiple sclerosis
- molecular docking
- inflammatory response
- electronic health record
- big data
- nitric oxide synthase
- endoplasmic reticulum stress
- anti inflammatory
- solid state
- data analysis
- deep learning
- arabidopsis thaliana