Double-Tetrahedral DNA Probe Functionalized Ag Nanorod Biointerface for Effective Capture, Highly Sensitive Detection, and Nondestructive Release of Circulating Tumor Cells.
Jinxiang LiYugang YuanHongyu GanChen DongBin CaoJin-Liang NiXueliang LiWenjie GuChunyuan SongLian-Hui WangPublished in: ACS applied materials & interfaces (2022)
Circulating tumor cells (CTCs) are indicative of tumorigenesis, metastasis, and recurrence; however, it is still a great challenge to efficiently analyze the extremely rare CTCs in peripheral blood. Herein, a novel nanobiointerface integrating high affinities of arrayed silver nanorods (Ag NRs) and double-tetrahedral DNA (DTDN) probes by a clever strategy is proposed for the efficient capture, highly sensitive detection, and nondestructive release of CTCs. Under the optimal conditions, the DTDN-probe-functionalized Ag NRs nanobiointerface can capture 90.2% of SGC-7901 cells in PBS, and the capture efficiency is 2.8 times and 50 times those of a DTDN-probe-functionalized Ag film and unfunctionalized Ag NRs, respectively, benefiting from the nanorough interface of the Ag NRs array and multivalent recognition of the DTDN probe. In addition, 93.4% of cells was released via Zn 2+ -assisted DNAzyme cleavage, and the viability of the postreleased CTCs is about 98.0%. The potential practicality of the nanobiointerface for testing CTCs in blood was further characterized by spiking SGC-7901 cells in leukocytes collected from human blood, and the results show that 83.8% capture efficiency, 91.2% release efficiency, and single-cell detection limit were achieved, which indicates that the nanobiointerface has great potential in clinical applications for reliable CTC analyses.
Keyphrases
- circulating tumor cells
- quantum dots
- sensitive detection
- circulating tumor
- induced apoptosis
- loop mediated isothermal amplification
- peripheral blood
- cell cycle arrest
- single cell
- living cells
- endoplasmic reticulum stress
- single molecule
- endothelial cells
- high throughput
- small molecule
- signaling pathway
- transcription factor
- cell proliferation
- mass spectrometry
- heavy metals
- risk assessment
- label free
- fluorescent probe
- induced pluripotent stem cells
- high density
- liquid chromatography