Naringenin protects swine testis cells from bisphenol A-induced apoptosis via Keap1/Nrf2 signaling pathway.
Huijie ChenJianqing ChenShi XuLu LiShi-Wen XuPublished in: BioFactors (Oxford, England) (2021)
Bisphenol A (BPA) has caused serious pathologies in varying organs of humans and animals, especially reproductive organs. Naringenin (NRG) is a flavanone compound that has shown protective effects against several environmental chemicals through suppression of oxidative stress and activation of nuclear factor erythroid 2-related factor 2 (Nrf2) pathway. Herein, we described the discovery path of NRG inhibition on apoptosis in BPA exposed swine testis (ST) cells through targeting Kelch-like ech-associated protein (Keap1). We found that NRG could specifically bound to the active residues of DGR domain in Keap1, thereby activating Nrf2 signaling pathway, and then increasing the levels of SOD, GPx and CAT, and finally inhibiting oxidative stress and mitochondrial apoptosis induced by BPA in ST cells. Altogether, our results showed that NRG inhibits oxidative stress and mitochondrial apoptosis induced by BPA in ST cells by targeting Keap1/Nrf2 signaling pathway, indicating that NRG could serve as an antagonistic therapy against BPA.
Keyphrases
- oxidative stress
- induced apoptosis
- signaling pathway
- nuclear factor
- diabetic rats
- pi k akt
- ischemia reperfusion injury
- dna damage
- endoplasmic reticulum stress
- cell cycle arrest
- protein protein
- epithelial mesenchymal transition
- toll like receptor
- cell death
- stem cells
- high throughput
- climate change
- inflammatory response
- cell proliferation
- cancer therapy
- single cell
- mesenchymal stem cells
- heat shock protein