DNA-Origami-Based Assembly of Au@Ag Nanostar Dimer Nanoantennas for Label-Free Sensing of Pyocyanin.
Vishaldeep KaurSwati TanwarGagandeep KaurTapasi SenPublished in: Chemphyschem : a European journal of chemical physics and physical chemistry (2020)
Early-stage detection of diseases caused by pathogens is a prerequisite for expedient patient care. Due to the limited signal-to-noise ratio, molecular diagnostics needs molecular signal amplification after recognition of the target molecule. In this present study, we demonstrate the design of plasmonically coupled bimetallic Ag coated Au nanostar dimers with controlled nanogap using rectangular DNA origami. We further report the utility of the designed nanostar dimer structures as efficient SERS substrate for the ultrasensitive and label-free detection of the pyocyanin molecule, which is a biomarker of the opportunistic pathogenic bacteria, Pseudomonas aeruginosa. The experimental results showed that the detection limit of pyocyanin with such nanoantenna based biosensor was 335 pM, which is much lower than the clinical range of detection. Thus, fast, sensitive and label-free detection of pyocyanin at ultralow concentration in an infected human body can pave a facile route for early stage warning for severe bacterial infections.
Keyphrases
- label free
- early stage
- pseudomonas aeruginosa
- single molecule
- quantum dots
- sensitive detection
- endothelial cells
- risk assessment
- reduced graphene oxide
- multidrug resistant
- biofilm formation
- drug resistant
- cell free
- escherichia coli
- antimicrobial resistance
- solid phase extraction
- amino acid
- liquid chromatography
- molecularly imprinted