Glochodpurnoid B from Glochidion puberum Induces Endoplasmic Reticulum Stress-Mediated Apoptosis in Colorectal Cancer Cells.
Yang TianRunzhu FanZhao YinYongping HuangDong HuangFangyu YuanAiping YinGui-Hua TangRong PuSheng YinPublished in: Molecules (Basel, Switzerland) (2023)
Glochidpurnoids A and B ( 1 and 2 ), two new coumaroyl or feruloyl oleananes, along with 17 known triterpenoids ( 3 - 19 ) were obtained from the stems and twigs of Glochidion puberum . Their structures were elucidated by extensive spectroscopic data analyses, chemical methods, and single crystal X-ray diffraction. All compounds were screened for cytotoxicity against the colorectal cancer cell line HCT-116, and 2 , 3 , 5 , 6 , 11 , and 17 showed remarkable inhibitory activities (IC 50 : 0.80-2.99 μM), being more active than the positive control 5-fluorouracil (5-FU). The mechanistic study of 2 , the most potent compound, showed that it could induce endoplasmic reticulum (ER) stress-mediated apoptosis and improve the sensitivity of HCT-116 cells to 5-FU.
Keyphrases
- induced apoptosis
- endoplasmic reticulum stress
- endoplasmic reticulum
- cell cycle arrest
- high resolution
- signaling pathway
- cell death
- oxidative stress
- pi k akt
- molecular docking
- electronic health record
- electron microscopy
- computed tomography
- magnetic resonance imaging
- magnetic resonance
- anti inflammatory
- contrast enhanced