HPLC-Based Automated Synthesis of Glycans in Solution.
Samira EscopyYashapal SinghKeith J StineAlexei V DemchenkoPublished in: Chemistry (Weinheim an der Bergstrasse, Germany) (2022)
As the 21 st century unfolds with rapid changes, new challenges in research and development emerge. These new challenges prompted us to repurpose our HPLC-A platform that was previously used in solid phase glycan synthesis to a solution phase batch synthesis described herein. The modular character of HPLC allows for implementing new attachments. To enable sequential synthesis of multiple oligosaccharides with the single press of a button, we supplemented our system with a four-way split valve and an automated fraction collector. This enabled the operator to load all reagents and all reactants in the autosampler, press the button to start the repetitive automation sequence, leave the lab, and upon return find products of multiple reactions ready for purification, analysis, and subsequent application.
Keyphrases
- ms ms
- simultaneous determination
- high performance liquid chromatography
- mass spectrometry
- solid phase extraction
- tandem mass spectrometry
- high throughput
- heart failure
- aortic valve
- deep learning
- high frequency
- quality improvement
- atrial fibrillation
- coronary artery disease
- high resolution
- left ventricular
- liquid chromatography