Application of Composite NanoMaterial to Determine Phenols in Wastewater by Solid Phase Micro Membrane Tip Extraction and Capillary Electrophoresis.

Composite nanoparticles were used in solid phase micro membrane tip extraction and capillary electrophoresis to determine phenol and p-amino-phenol in wastewater. The optimized conditions were 100 g/L concentration, 40 min contact time, 11 pH, 5 mg/mL nanoparticles amounts, 60 min desorption time, 9 desorption pH and 298 K temperature. Capillary electrophoresis conditions were phosphate buffer (15 mM, pH 7.0) background electrolyte, 18 kV applied voltage, 214 nm UV detection, 30 s sample loading at 23 ± 1 °C. The maximum percent uptakes of p-amino-phenol and phenol were 80.0 and 85.0%. High ratio recoveries of p-amino-phenol and phenol from nanomaterial were 99.0 and 98. Consequently, the actual extractions of p-amino-phenol and phenol from wastewater were 79.2 and 83.30 percent. The migration times of phenol and p-amino-phenol and were 9.0 and 12.0 min. The detection limits of phenol and p-amino-phenols were 0.1 and 0.2 µg/L after extraction and CE. Therefore, this combination of solid phase micro membrane tip extraction and capillary electrophoresis may be considered as the ideal one for monitoring of toxic phenol and p-amino-phenol in water sample.