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Protein-free media for cardiac differentiation of hPSCs in 2000 mL suspension culture.

Nils KriedemannFelix MansteinCarlos A Hernandez-BautistaKevin UllmannWiebke TriebertAnnika FrankeMira MertensInês Carvalheira Arnaut Pombeiro SteinAndreas LefflerMerlin WitteTamari AskuravaVeronika FrickeIna GruhBirgit PiepKathrin KowalskiTheresia KraftRobert Zweigerdt
Published in: Stem cell research & therapy (2024)
This study promotes the robustness and upscaling of the cardiomyogenic differentiation process, substantially reduces media costs, and provides an important step toward the clinical translation of hPSC-CMs for heart regeneration.
Keyphrases
  • stem cells
  • heart failure
  • atrial fibrillation
  • amino acid
  • protein protein