Confinement of Triple-Enzyme-Involved Antioxidant Cascade in Two-Dimensional Nanostructure.
Adél SzerlauthÁrpád VargaTamara MadácsyDániel SebőkSahra BashiriMariusz SkwarczynskiIstvan TothJózsef MaléthIstvan SzilagyiPublished in: ACS materials letters (2023)
Application of antioxidant enzymes in medical or industrial processes is limited due to their high sensitivity to environmental conditions. Incorporation of such enzymes in nanostructures provides a promising route to obtain highly efficient and robust biocatalytic system to scavenge reactive oxygen species (ROS). Here, this question was addressed by confinement of superoxide dismutase (SOD), horseradish peroxidase (HRP), and catalase (CAT) enzymes into nanostructures containing polyelectrolyte building blocks (alginate (Alg) and trimethyl chitosan (TMC)) and delaminated layered double hydroxide (dLDH) nanoparticle support. The nanocomposite possessed excellent structural and colloidal stability, while antioxidant tests revealed that the enzymes remained active upon immobilization and the developed composite greatly reduced intracellular oxidative stress in two-dimensional cell cultures. Moreover, it effectively prevented hydrogen peroxide-induced double stranded DNA breaks, which is a common consequence of oxidative stress. The results provide important tools to design complex nanostructures with multienzymatic antioxidant activities for ROS scavenging.
Keyphrases
- oxidative stress
- hydrogen peroxide
- reactive oxygen species
- diabetic rats
- highly efficient
- dna damage
- anti inflammatory
- induced apoptosis
- ischemia reperfusion injury
- nitric oxide
- cell death
- single cell
- healthcare
- heavy metals
- high glucose
- reduced graphene oxide
- cell free
- nucleic acid
- quantum dots
- wound healing
- risk assessment
- mesenchymal stem cells
- binding protein
- gold nanoparticles
- carbon nanotubes
- mass spectrometry
- stress induced
- drug induced
- high resolution
- bone marrow
- life cycle
- tissue engineering