Detection of Microsporum canis and Trichophyton mentagrophytes by loop-mediated isothermal amplification (LAMP) and real-time quantitative PCR (qPCR) methods.

These findings demonstrate that the LAMP and multiplex qPCR methods targeting CHS-1 gene developed in this study can be used both for point-of-care testing and in the laboratory for detecting M. canis and T. mentagrophytes with high specificity and sensitivity with an internal control.