A dynamic atlas of immunocyte migration from the gut.
Silvia Galvan-PenaYangyang ZhuBola S HannaDiane MathisChristophe BenoistPublished in: Science immunology (2024)
Dysbiosis in the gut microbiota affects several systemic diseases, possibly by driving the migration of perturbed intestinal immunocytes to extraintestinal tissues. Combining Kaede photoconvertible mice and single-cell genomics, we generated a detailed map of migratory trajectories from the colon, at baseline, and in several models of intestinal and extraintestinal inflammation. All lineages emigrated from the colon in an S1P-dependent manner. B lymphocytes represented the largest contingent, with the unexpected circulation of nonexperienced follicular B cells, which carried a gut-imprinted transcriptomic signature. T cell emigration included distinct groups of RORγ + and IEL-like CD160 + subsets. Gut inflammation curtailed emigration, except for dendritic cells disseminating to lymph nodes. Colon-emigrating cells distributed differentially to distinct sites of extraintestinal models of inflammation (psoriasis-like skin, arthritic synovium, and tumors). Thus, specific cellular trails originating in the gut and influenced by microbiota may shape peripheral immunity in varied ways.
Keyphrases
- single cell
- oxidative stress
- dendritic cells
- rna seq
- lymph node
- induced apoptosis
- peripheral blood
- high throughput
- depressive symptoms
- immune response
- adipose tissue
- cell cycle arrest
- regulatory t cells
- type diabetes
- early stage
- soft tissue
- sentinel lymph node
- endoplasmic reticulum stress
- high resolution
- liquid chromatography
- signaling pathway
- wild type
- pi k akt
- chemotherapy induced