Ultrasensitive detection of lipoarabinomannan with plasmonic grating biosensors in clinical samples of HIV negative patients with tuberculosis.
Aaron WoodSyed BarizuddinCharles M DarrCherian J MathaiAlexey BallKyle MinchAkos SomoskoviBeston HamasurJohn T ConnellyBernhard WeiglAlfred AndamaAdithya CattamanchiKeshab GangopadhyaySangho BokShubhra GangopadhyayPublished in: PloS one (2019)
Single molecule FLISA by plasmonic grating demonstrated the ability to quantify tuberculosis LAM from complex urine samples of patients from a high endemic setting with negligible interference from the complex media itself. Moreover, agreement with patient diagnoses by gold standard testing suggests that single molecule FLISA could be used as a highly sensitive test to diagnose tuberculosis noninvasively.
Keyphrases
- single molecule
- living cells
- mycobacterium tuberculosis
- hiv aids
- atomic force microscopy
- end stage renal disease
- label free
- pulmonary tuberculosis
- antiretroviral therapy
- newly diagnosed
- ejection fraction
- chronic kidney disease
- hiv infected
- human immunodeficiency virus
- peritoneal dialysis
- hepatitis c virus
- hiv positive
- prognostic factors
- gold nanoparticles
- case report
- fluorescent probe
- south africa
- high resolution
- loop mediated isothermal amplification
- patient reported