Live nanoscopic to mesoscopic topography reconstruction with an optical microscope for chemical and biological samples.
Olivier TheodolyNicolas Garcia-SeydaFréderic BeduXuan LuoSylvain GabrieleTâm MignotJoanna GiermanskaJean-Paul ChapelMélinda MétivierMarie-Pierre ValignatPublished in: PloS one (2018)
Macroscopic properties of physical and biological processes like friction, wetting, and adhesion or cell migration are controlled by interfacial properties at the nanoscopic scale. In an attempt to bridge simultaneously investigations at different scales, we demonstrate here how optical microscopy in Wet-Surface Ellipsometric Enhanced Contrast (Wet-SEEC) mode offers imaging and measurement of thin films at solid/liquid interfaces in the range 1-500 nm with lateral optical resolution. A live, label-free and noninvasive methodology integrated with microfluidic devices allowed here characterization of polymers and proteins patterns together with corresponding phenotypes of living cells.
Keyphrases
- label free
- high resolution
- cell migration
- living cells
- high speed
- single molecule
- fluorescent probe
- ionic liquid
- high throughput
- magnetic resonance
- physical activity
- mental health
- mass spectrometry
- minimally invasive
- magnetic resonance imaging
- single cell
- staphylococcus aureus
- circulating tumor cells
- contrast enhanced
- electron transfer