Whole exome sequencing analysis of urine trans-renal tumour DNA in metastatic colorectal cancer patients.

Giovanni CrisafulliBenedetta MussolinAndrea CassingenaMonica MontoneAlice BartoliniLudovic BaraultAntonia MartinettiFederica MoranoFilippo PietrantonioAndrea Sartore-BianchiSalvatore SienaFederica Di NicolantonioSilvia MarsoniAlberto BardelliGiulia Siravegna

Published in: ESMO open (2020)

With current technologies, WES analysis of trtDNA is feasible in a small fraction of mCRC patients. Tumour-related genetic information is mainly present in low molecular weight DNA fragments. Although the limited amounts of trtDNA poses analytical challenges, enrichment of low molecular weight DNAs and optimised computational tools can improve the detection of tumour-specific genetic information in urine.

Keyphrases

metastatic colorectal cancer
end stage renal disease
circulating tumor
genome wide
newly diagnosed
single molecule
chronic kidney disease
ejection fraction
cell free
peritoneal dialysis
healthcare
gene expression
dna methylation
social media
circulating tumor cells
patient reported