Two new bibenzyl methylglucosides as SIRT3 activators obtained through microbial transformation.

Microbial transformation of dihydroresveratrol (DHRSV) using Beauveria bassiana has produced two new methylglucosylated derivatives of DHRSV ( 1 and 2) , whose structures were characterized as 4'- O -(4″- O -methyl- β -D-glucopyranosyl)-dihydroresveratrol (4'- O -MG DHRSV, 1 ) and 3- O -(4″- O -methyl- β -D-glucopyranosyl)-dihydroresveratrol (3- O -MG DHRSV, 2 ) on the basis of spectroscopic methods. They showed moderate SIRT3 agonistic activity, and compound 2 exhibited the best deacetylation of 406.63% at 10 μM. The activity of 2 increased by 3.12-fold compared with that of DHRSV, since 2 performed better in molecular docking assay (GScore -8.445).