Rod-shaped gold nanoparticles exert potent candidacidal activity and decrease the adhesion of fungal cells.
Ewelina PiktelŁukasz SuprewiczJoanna DepciuchMateusz CieślukSylwia ChmielewskaBonita DurnaśGrzegorz KrólTomasz WollnyPiotr DeptułaJan KochanowiczAlina KułakowskaKrzysztof FiedorukAlexey MaximenkoMagdalena Parlińska-WojtanRobert BuckiPublished in: Nanomedicine (London, England) (2020)
Aim: To investigate the fungicidal activity of rod-shaped gold nanoparticles (AuR NPs) against Candida strains isolated from hematooncological patients and representative strains of filamentous fungi. Methods: Colony-counting assays, colorimetric and fluorometric methods and atomic force microscopy were employed. Results: AuR NPs were characterized by their potent fungicidal activity against all tested isolates, regardless of the species or drug susceptibility, at concentrations that are nontoxic to the host cells. The membrane-permeabilizing properties of AuR NPs and induction of reactive oxygen species were recognized as crucial for fungicidal activity. Conclusions: The results provide a rationale for the development of nonspherical Au NPs as effective antifungals or drug-delivery carriers to improve therapy for fungal infections.
Keyphrases
- gold nanoparticles
- induced apoptosis
- drug delivery
- atomic force microscopy
- escherichia coli
- end stage renal disease
- reduced graphene oxide
- cell cycle arrest
- newly diagnosed
- chronic kidney disease
- high speed
- emergency department
- clinical trial
- ejection fraction
- oxidative stress
- sensitive detection
- high resolution
- high throughput
- cell death
- hydrogen peroxide
- nitric oxide
- candida albicans
- prognostic factors
- patient reported outcomes
- cancer therapy
- mass spectrometry
- single cell
- quantum dots
- aqueous solution
- pseudomonas aeruginosa
- simultaneous determination
- living cells