Periradicular tissue fluid-derived biomarkers for apical periodontitis: an in vitro methodological & in vivo cross-sectional study.

Optimised PTF sampling parameters were identified in this study. When applied clinically, high-throughput proteomic analyses revealed complex interconnected networks of potential biomarkers. TNFSF-12 discriminated periradicular disease from health the greatest however, clustering analytes further improved diagnostic accuracy. Additional independent investigations are required to validate these findings.