Ballistic gelatin has been extensively used in ballistics research for decades, but calibration standards were established on limited datasets, and only few studies have attempted to recreate these experiments with biological tissues. Recent studies have demonstrated better biofidelity with 20% ordnance ballistic gelatin, but researchers have discredited the use of synthetic gelatin claiming different behavior than ordnance gelatin. To investigate the use of synthetic clear gelatin as an acceptable surrogate of biological tissue, depth of penetration was compared between low-velocity impacts of various projectiles into porcine tissue (n = 192), post-mortem human subjects (n = 29), and Clear Ballistics synthetic gelatin (n = 39). The predicted depth of penetration of the 0.177" steel BB (38.1 mm) was consistent with the manufacturer's calibration standard (31.75-44.45 mm) and within calibration bounds of recently proposed empirical equations. Compared to impacts in biological tissue, synthetic gelatin demonstrated the least variability in depth of penetration (R 2 = 0.96). Using ANCOVA, velocity was a significant covariate (p < 0.001), and there were no significant differences in normalized depth of penetration over density between porcine tissue, post-mortem human subjects, and 20% synthetic gelatin (p = 0.22). Ultimately, this study confirmed the use of 20% synthetic gelatin as an acceptable tissue simulant using standard calibration methods for use in future ballistic studies.